NikR is a ribbon-helix-helix DNA-binding protein

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NikR is a ribbon-helix-helix DNA-binding protein.

Escherichia coli NikR, a repressor with homologs in other bacteria and archaea, was identified as a potential new member of the ribbon-helix-helix (beta-alpha-alpha) family of transcription factors in profile based sequence searches and in structure prediction experiments. Biophysical and biochemical characterization of the N-terminal domain of NikR show that it has many features expected of a ...

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Agrobacterium tumefaciens VirC2 enhances T-DNA transfer and virulence through its C-terminal ribbon-helix-helix DNA-binding fold.

Agrobacterium tumefaciens VirC2 stimulates processing of single-stranded T-DNA that is translocated into plants to induce tumor formation, but how VirC2 functions is unclear. Here, we report the 1.7-A X-ray crystal structure of its trypsin-resistant C-terminal domain, VirC2(82-202), which reveals a form of the ribbon-helix-helix (RHH) DNA-binding fold contained within a single polypeptide chain...

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The Saccharomyces cerevisiae CDC43 gene product is involved in establishing cell polarity during the cell-division cycle. When grown at restrictive temperatures, temperature-sensitive cdc43 mutants are unable to form buds and display delocalized cell-surface deposition [Adams et al., J. Cell Biol. (1990) in press]. We have isolated a cdc43-complementing plasmid from a yeast genomic-DNA library ...

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Commentary Helix is a helix is a helix ?

There is much activity directed at quantifying the contribution of specific interactions to the stabilization of protein structure. The paper by Myers et al. (1) in this issue of the Proceedings addresses a central and much studied issue: The experimental measure of the intrinsic a-helix forming propensities of the amino acids. What is an intrinsic a-helix forming propensity? The idea that the ...

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ID 4 ( inhibitor of DNA binding 4 , dominant negative helix - loop - helix protein )

ID4 is one of the members of the ID gene family: "Inhibitors of DNA binding". They are transcription factors which act as transcription inhibitory proteins. They are basic helix-loop-helix (bHLH) proteins which contain the bHLH dimerization domain, but lack the DNA binding domain. They are able to form heterodimers with other bHLH proteins, but inhibit the DNA binding, inactivating the process....

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ژورنال

عنوان ژورنال: Protein Science

سال: 2008

ISSN: 0961-8368

DOI: 10.1110/ps.8.11.2494